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KMID : 0881720180330030193
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 3 p.193 ~ p.199
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Production and Characterization of Monoclonal Antibodies Specific to PAT Protein Expressed in Genetically Modified Herbicide-Resistance Maize
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Kim Sol-A
Lee Jeong-Eun
Shim Won-Bo
Kang Sung-Jo
Chung Duck-Hwa
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Abstract
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In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.
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KEYWORD
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GM maize , PAT protein , Hybridoma cell , Monoclonal antibody
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